Chromatin Immunoprecipitation:Article Title: Independence between pre-mRNA splicing and DNA methylation in an isogenic minigene resource.
Article Snippet: .. Nuclear pellets were resuspended in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris–HCl; pH 8.0), supplemented with Halt protease inhibitors (Thermo Scientific), and chromatin sheared to an average size between 150 and 500 bp by sonication (Bioruptor Twin, Diagenode). .. Chromatin preparations were cleared by centrifugation at 20 000 × g for 10 min at 4 ◦C and protein content measured by BCA assay (Thermo Scientific).
Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
Article Snippet: .. Nuclear pellets were resuspended to a concentration of 200 million cells/mL in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris–HCl; pH 8.0), supplemented with Halt protease inhibitors (Thermo Scientific), and chromatin sheared to an average size between 150 and 400 bp by sonication (Bioruptor Twin, Diagenode). ..
Article Title: TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide
Article Snippet: .. Nuclear pellets were resuspended at a concentration of 2E8 cells/ml in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris– HCl; pH 8.0) supplemented with protease (Thermo Scientific, #78430) and phosphatase (Calbiochem, #524627) inhibitors. .. Chromatin was sheared into 150-400 bp fragments by sonication (Bioruptor Twin, Diagenode).
Article Title: Multiple strategies to resolve the conflict between replication and transcription in mammalian cells.
Article Snippet: .. Nuclei were pelleted, resuspended in ChIP sonication buffer [50 mM HEPES pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Nadeoxycholate, 0.1% sodium dodecyl sulfate (SDS)] supplemented with protease and phosphatase inhibitors, and sonicated using a probe-based sonicator (FB120 with a CL-18 probe; Thermo Fisher) at 25% amplitude with 30-s pulses at 30-s intervals for a total of 3 min. Sonicated chromatin was stored until immunoprecipitation. ..
Article Title: TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide
Article Snippet: .. Nuclear pellets were resuspended at a concentration of 2E8 cells/ml in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris– HCl; pH 8.0) supplemented with protease (Thermo Scientific, #78430) and phosphatase (Calbiochem, #524627) inhibitors. .. Chromatin was sheared into 150-400 bp fragments by sonication (Bioruptor Twin, Diagenode).
Article Title: Multiple strategies to resolve the conflict between replication and transcription in mammalian cells
Article Snippet: .. Nuclei were pelleted, resuspended in ChIP sonication buffer [50 mM HEPES pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% sodium dodecyl sulfate (SDS)] supplemented with protease and phosphatase inhibitors, and sonicated using a probe-based sonicator (FB120 with a CL-18 probe; Thermo Fisher) at 25% amplitude with 30-s pulses at 30-s intervals for a total of 3 min. Sonicated chromatin was stored until immunoprecipitation. ..
Sonication:Article Title: Independence between pre-mRNA splicing and DNA methylation in an isogenic minigene resource.
Article Snippet: .. Nuclear pellets were resuspended in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris–HCl; pH 8.0), supplemented with Halt protease inhibitors (Thermo Scientific), and chromatin sheared to an average size between 150 and 500 bp by sonication (Bioruptor Twin, Diagenode). .. Chromatin preparations were cleared by centrifugation at 20 000 × g for 10 min at 4 ◦C and protein content measured by BCA assay (Thermo Scientific).
Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
Article Snippet: .. Nuclear pellets were resuspended to a concentration of 200 million cells/mL in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris–HCl; pH 8.0), supplemented with Halt protease inhibitors (Thermo Scientific), and chromatin sheared to an average size between 150 and 400 bp by sonication (Bioruptor Twin, Diagenode). ..
Article Title: TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide
Article Snippet: .. Nuclear pellets were resuspended at a concentration of 2E8 cells/ml in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris– HCl; pH 8.0) supplemented with protease (Thermo Scientific, #78430) and phosphatase (Calbiochem, #524627) inhibitors. .. Chromatin was sheared into 150-400 bp fragments by sonication (Bioruptor Twin, Diagenode).
Article Title: Multiple strategies to resolve the conflict between replication and transcription in mammalian cells.
Article Snippet: .. Nuclei were pelleted, resuspended in ChIP sonication buffer [50 mM HEPES pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Nadeoxycholate, 0.1% sodium dodecyl sulfate (SDS)] supplemented with protease and phosphatase inhibitors, and sonicated using a probe-based sonicator (FB120 with a CL-18 probe; Thermo Fisher) at 25% amplitude with 30-s pulses at 30-s intervals for a total of 3 min. Sonicated chromatin was stored until immunoprecipitation. ..
Article Title: TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide
Article Snippet: .. Nuclear pellets were resuspended at a concentration of 2E8 cells/ml in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris– HCl; pH 8.0) supplemented with protease (Thermo Scientific, #78430) and phosphatase (Calbiochem, #524627) inhibitors. .. Chromatin was sheared into 150-400 bp fragments by sonication (Bioruptor Twin, Diagenode).
Article Title: Multiple strategies to resolve the conflict between replication and transcription in mammalian cells
Article Snippet: .. Nuclei were pelleted, resuspended in ChIP sonication buffer [50 mM HEPES pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% sodium dodecyl sulfate (SDS)] supplemented with protease and phosphatase inhibitors, and sonicated using a probe-based sonicator (FB120 with a CL-18 probe; Thermo Fisher) at 25% amplitude with 30-s pulses at 30-s intervals for a total of 3 min. Sonicated chromatin was stored until immunoprecipitation. ..
Concentration Assay:Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
Article Snippet: .. Nuclear pellets were resuspended to a concentration of 200 million cells/mL in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris–HCl; pH 8.0), supplemented with Halt protease inhibitors (Thermo Scientific), and chromatin sheared to an average size between 150 and 400 bp by sonication (Bioruptor Twin, Diagenode). ..
Article Title: TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide
Article Snippet: .. Nuclear pellets were resuspended at a concentration of 2E8 cells/ml in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris– HCl; pH 8.0) supplemented with protease (Thermo Scientific, #78430) and phosphatase (Calbiochem, #524627) inhibitors. .. Chromatin was sheared into 150-400 bp fragments by sonication (Bioruptor Twin, Diagenode).
Article Title: TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide
Article Snippet: .. Nuclear pellets were resuspended at a concentration of 2E8 cells/ml in ChIP sonication buffer (1% SDS, 10 mM EDTA, 50 mM Tris– HCl; pH 8.0) supplemented with protease (Thermo Scientific, #78430) and phosphatase (Calbiochem, #524627) inhibitors. .. Chromatin was sheared into 150-400 bp fragments by sonication (Bioruptor Twin, Diagenode).
Immunoprecipitation:Article Title: Multiple strategies to resolve the conflict between replication and transcription in mammalian cells.
Article Snippet: .. Nuclei were pelleted, resuspended in ChIP sonication buffer [50 mM HEPES pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Nadeoxycholate, 0.1% sodium dodecyl sulfate (SDS)] supplemented with protease and phosphatase inhibitors, and sonicated using a probe-based sonicator (FB120 with a CL-18 probe; Thermo Fisher) at 25% amplitude with 30-s pulses at 30-s intervals for a total of 3 min. Sonicated chromatin was stored until immunoprecipitation. ..
Article Title: Multiple strategies to resolve the conflict between replication and transcription in mammalian cells
Article Snippet: .. Nuclei were pelleted, resuspended in ChIP sonication buffer [50 mM HEPES pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% sodium dodecyl sulfate (SDS)] supplemented with protease and phosphatase inhibitors, and sonicated using a probe-based sonicator (FB120 with a CL-18 probe; Thermo Fisher) at 25% amplitude with 30-s pulses at 30-s intervals for a total of 3 min. Sonicated chromatin was stored until immunoprecipitation. ..
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